Methodology & Resources

Transgenic mouse models, conditional knockout, segment-specific invalidation
Immortalized cell lines and primary cell culture systems
Manipulation of gene expression in cell lines and primary cultures
Promoter analysis (in silico, in vitro)
In situ hybridization, advanced quantitative RT-PCR
Immunoblotting, immunoprecipitation, and immunohisto-/cyto-chemistry
Intracellular distribution studies: subcellular fractionation, immunogold, biotinylation
Transport studies in cells and native tissues (Ussing chamber)
Mouse phenotyping: metabolic cages, special diets, pharmacology interventions
Multisystemic phenotyping: cardiovascular, osmoregulation & thirst
Biochemical profiling on dedicated platform optimalized for rodent samples
Development and automation of ELISA
Water and solute transport in mouse model of peritoneal dialysis
Biobanking: end-stage kidney samples (300+); kidney biopsies (1000+); urine samples from isolated populations (6000); peritoneal biopsies (100+)
DNA cohorts: ADPKD (300); rare inherited kidney disorders (200); renal transplant (300); peritoneal dialysis (800)
EU-funded programs: EUROSPAN and EURenOmics consortia