RNA and cDNA quality control
NanoDrop ND-1000 Spectrophotometer
Full-spectrum UV-Vis absorbance analysis (220 nm to 750 nm) using a single microliter sample. Large dynamic range (2-3700 ng/µl of dsDNA).
Quality assessment of primary sample, amplified sample and labeled sample :
Agilent Bioanalyser system
Analysis of RNA using microfluidic Lab-on-a-chip technology (12 samples in one run). It offers fast, reproducible and high quality digital data with minimal sample and reagent consumption.
Quality assessment of RNA integrity (RIN) by creation of RNA peak profiles and automatic calculation of the ratio of ribosomal bands in total RNA samples.
Hybridization and scanning
- DNA Microarray Hybridization Oven
- Agilent DNA microarray Scanner G2565B
Simultaneous two-colors scanning at either 5 or 10 micro resolution. The scanning system can read up to 48 glass slide microarrays. Extended dynamic range of fluorescence with high sensitivity scanning without saturation.
Computers and bioinformatics
Servers : data-storage, sate management, image analysis, and data analysis.
Software : Feature extraction : image analysis and normalization of Agilent microarrays; GeneSpring GX : visualization and analysis of expression data (statistical analysis, clustering, pathway mapping, ...).