Exploring platelet signalling and metabolism to identify new predictive markers of arterial thrombosis
The major physiological role of platelets is to ensure integrity of vessels, primary hemostasis and coagulation. Platelet activation and aggregation are also involved in pathological processes like arterial thrombosis, fearsome complication of atherosclerosis, which can lead to coronary occlusion and myocardial infarction. Cardiovascular diseases have become the leading cause of death in industrialized countries and are therefore a key public health issue.
Research in our group aims at identifying new molecular mechanisms responsible for controlling platelet function and metabolism. The experimental approach is based on preparation and ex vivo treatment of human platelets taken from healthy volunteers, or platelets from mice, the latter providing genetic evidences through the use of transgenic models. The mixed basic/clinical nature of our team also highlights a translational approach that supports experimental data obtained in the lab by clinical evidences obtained in the patient.
In addition to measurement of platelet aggregation, our experimental approach involves the use of various biochemical and molecular biology techniques (expression and phosphorylation of proteins, enzymatic assays, evaluation of metabolic processes, FACS, PCR and qRT-PCR, microarray analysis), microscopy (thrombus analysis through intravital microscopy) and physiology (animal model of atherothrombosis).
Exploring platelet signalling and metabolism to identify new predictive markers of arterial thrombosis
Research in our group aims at identifying new molecular mechanisms responsible for controlling platelet function and metabolism. The experimental approach is based on preparation and ex vivo treatment of human platelets taken from healthy volunteers, or platelets from mice, the latter providing genetic evidences through the use of transgenic models. The mixed basic/clinical nature of our team also highlights a translational approach that supports experimental data obtained in the lab by clinical evidences obtained in the patient.
In addition to measurement of platelet aggregation, our experimental approach involves the use of various biochemical and molecular biology techniques (expression and phosphorylation of proteins, enzymatic assays, evaluation of metabolic processes, FACS, PCR and qRT-PCR, microarray analysis), microscopy (thrombus analysis through intravital microscopy) and physiology (animal model of atherothrombosis).
