FRIDA (FRamework for Image Dataset Analysis) is a very simple free software, based on JAVA language, allowing quantification of stained area.
The TMAJ software allowing tissue microarray analysis, integrated into FRIDA, is also freely accessible.
Application example
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Collagen fibers analysis according to their polarization colors
5 µm paraffin section of heart - picrosirius red staining
image acquired in polarized light (Axioskop40) - x20 objective