Antigen retrieval solutions
- Citrate (pH5.7)
| Stock solution (10x) | |
| Citric acid | 3.78 g |
| Sodium citrate dihydrate (C6H5Na3O7.2H20) | 24.12 g |
| dist H2O | ad 1 L |
| Adjust to pH 5.7 | |
| Store 20 ml aliquots at -20°C |
| Working solution | |
| Stock solution | 20 ml |
| dist H2O | 180 ml |
| Triton X-100 20% | 540 µl |
- EDTA (1 mM, pH 8,0):
| EDTA (C10H14N2O8Na2.2H2O) | 0.372 g |
| dist H2O | ad 1 L |
| Adjust to pH 8.0 | |
| Store at 4°C | |
| Extemporaneously: Triton X-100 20% | 540 µl |
- Tris-EDTA (10 mM Tris/1 mM EDTA, pH 9.0):
| Tris Base (C4H11NO3) | 12.1 g |
| EDTA (C10H14N2O8Na2.2H2O) | 3.7 g |
| dist H2O | ad 1 L |
| Adjust to pH 9.0 | |
| Store at 4°C | |
| Extemporaneously: Triton X-100 20% | 540 µl |
Human Protein Atlas (antibodies + protein and RNA expression profiles)
NordiQC (Immunohistochemical quality control)
ImmunoHistoChemistry Frequently Asked Questions (library of antibodies used in pathology)
Antibody-Adviser (academic forum)
UCL spin-off SynAbs 
is specialized in the production of:
control isotypes
monoclonal secondary antibodies: anti-mouse, rat, human, pig, horse, chicken Ig
control Ig (anti-DNP,...)
anti-cytokines antibodies
Based on Jockusch et al. (J Histochem Cytochem 2003 51: 401-404)
