Publications

2017

D. Alsteens, H.E. Gaub, R. Newton, M. Pfreundschuh, C. Gerber, D.J. Müller, Atomic force microscopy based characterization and design of biointerfaces, Nat. Rev. Mater., 2 (2017).
Y.F. Dufrêne, T. Ando, R. Garcia, D. Alsteens, D. Martinez-Martin, A. Engel, C. Gerber, D.J. Müller, Imaging modes of atomic force microscopy for application in molecular and cell biology, Nat. Nanotechnol., 12 (2017), 395-307.
D. Alsteens, D.J. Müller, Y.F. Dufrêne, Multiparametric atomic force microscopy imaging of biomolecular and cellular systems, Acc. Chem. Res., 50 (2017), 924-931.
D. Alsteens, R. Newton, R. Schubert, D Martinez-Martin, M. Delguste, B. Roska, DJ Müller, Nanomechanical mapping of first binding steps of a virus to animal cells, Nat. Nanotechnol., 12 (2017), 177-183.

2016

P. Herman, C. Valotteau, G. Pietrocola, S. Rindi, D. Alsteens, T.J. Foster, P. Speziale, Y.F. Dufrêne, Mechanical Strength and Inhibition of the Staphylococcus aureus Collagen-Binding Protein Cna, mBio., 7 (2016).
M. Kragel, R. Schubert, H. Karsjens , S. Otter, B. Bartosinska, K. Jeruschke, J. Weiss, C. Chen, D. Alsteens, O. Kuss, S. Speier, D. Eberhard, D.J. Müller, E. Lammert, The biomechanical properties of an epithelial tissue determine the location of its vasculature, Nat. Commun., 7 (2016).
D. Alsteens, Study of membrane receptors by atomic force microscopy, Med./Sci., 5 (2016), 433-435.

2015

M. Pfreundschuh, D. Alsteens, R. Wieneke, C. Zhang, S. Coughlin, R. Tampé, B. Kobilka, D.J. Müller, Imaging single G-protein coupled receptors while quantifying their binding to two different ligands, Nat. Commun., 6 (2015).
D. Alsteens, M. Pfreundschuh, C. Zhang, P. Spoerri, S. Coughlin, B. Kobilka, D.J. Müller, Imaging of G-protein coupled receptors while quantifying their ligand-binding free energy landscape, Nat. Methods., 12 (2015), 845-851.
R. Petrosyan, C. Bippes, S.Walheim, D. Harder, D. Fotiadis, T. Schimmel, D. Alsteens, D.J. Müller, Single-molecule force spectroscopy of membrane proteins from membranes freely spanning across nanoscopic pores, Nano Lett., 15 (2015), 3624-3633.
S. El-Kirat-Chatel, A. Beaussart, S. Derclaye, D.Alsteens, S. Kucharikova, P. Van Dijck, Yves Dufrêne, Force nanoscopy of hydrophobic interactions in the fungal pathogen Candida glabrata, ACS Nano., 9 (2015), 1648-1655.
D. Alsteens, S. Tay, D.J. Müller, Towards high-throughput biomechanical phenotyping of single molecules (News and Views), Nat. Methods., 12 (2015), 45-46.

2014

M. Pfreundschuh, D. Alsteens, M. Hilbert, M.O. Steinmetz, D.J. Müller, Localizing chemical groups while imaging single native proteins by high-resolution AFM, Nano Lett., 14 (2014), 2957-2964.
E. Dague, G. Genet, V. Lachaize, C. Guilbeau-Frugier, J. Fauconnier, C. Mias, B. Payre, L. Chopinet, D. Alsteens, S. Kasas, C. Severac, J. Thireau, C. Heymes, B. Honton, A. Lacampagne, A. Pathak, J.M. Senard, C. Gales , Atomic force and electron microscopic-based study of sarcolemmal surface of living cardiomyocytes unveils unexpected mitochondrial shift in heart failure, J. Mol. Cell. Cardiol., 74 (2014), 162-172.
Beaussart, S. El-Kirat-Chatel, R.M.A Sullan, D. Alsteens, P. Herman, S. Derclaye, Y.F. Dufrêne, Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy, Nat. Protocols., 9 (2014), 1049-1055.

2013

D. Alsteens, H. Trabelsi, P. Soumillion, Y.F. Dufrêne, Multiparametric atomic force microscopy imaging of single bacteriophage extruding from living bacteria, Nat. Commun., 4 (2013).
D. Alsteens, P. Van Dijck, P.N. Lipke, Y.F. Dufrêne , Quantifying the forces driving cell-cell adhesion in a fungal pathogen, Langmuir., 29 (2013), 13473-13480.
D. Alsteens, A. Beaussart, S. El-Kirat-Chatel, R.M.A. Sullan, Y.F. Dufrêne, Atomic force microscopy: a new look at pathogens, PLoS Pathogens., 9 (2013).
Y.F. Dufrêne, D. Martinez-Martin, I. Medalsy, D. Alsteens, D.J. Müller, Multiparametric imaging of biological systems by force-distance curve-based AFM, Nat. Methods., 10 (2013), 847-854.
D.Alsteens, N. Martinez, M. Jamin, F. Jacob-Dubuisson, Sequential unfolding of beta helical protein by single-molecule atomic force microscopy, PLoS One., 8 (2013).
D. Alsteens, V. Aimanianda, P. Hegde, S. Pire, R. Beau, J. Bayry, J.-P. Latge, Y.F. Dufrêne, Unraveling the nanoscale surface properties of Chitin synthase mutants of Aspergillus fumigatus and their biological implications, Biophys. J., 105 (2013), 320–327.
D. Alsteens, A. Beaussart, S. Derclaye, S. El-Kirat-Chatel, H.R. Park, P.N. Lipke, Y.F. Dufrêne, Single-cell force spectroscopy of Als-mediated fungal adhesion, Analytical Methods., 5 (2013), 3657-3662.
S. El-Kirat-Chatel, A. Beaussart, D. Alsteens, A. Sarazin, T. Jouault, Y.F. Dufrêne, Single-molecule analysis of the major glycopolymers of pathogenic and non-pathogenic yeast cells, Nanoscale., 5 (2013), 4855-4863.
D. Alsteens, Role of the amyloid region in the formation and propagation of Als adhesive nanodomains on Candida albicans, Proceedings of SPIE., 8590 (2013).
A. Beaussart, S. El-Kirat-Chatel, P. Herman, D. Alsteens, J. Mahillon, P. Hols, Y.F. Dufrêne, Single-cell force spectroscopy of probiotic bacteria, Biophys. J., 104 (2013), 1886-1892.
Tripathi, A. Beaussart, D. Alsteens, V. Dupres, I. Claes I. Von Ossowski, W.M. De Vos, A. Palva, S. Lebeer, J. Vanderleyden, Y.F. Dufrêne, Adhesion and nanomechanics of pili from the probiotic Lactobacillus rhamnosus GG, ACS Nano., 7 (2013), 3685-3697.
S. El-Kirat-Chatel, A. Beaussart, D. Alsteens, D.N. Jackson, P.N. Lipke, Y.F. Dufrêne, Nanoscale analysis of caspofungin-induced cell surface remodeling in Candida albicans, Nanoscale., 5 (2013), 1105-1115.

2012

D. Alsteens, V. Dupres, S. Yunus, J.P. Latgé, J.J. Heinisch, Y.F. Dufrêne, High-resolution imaging of chemical and biological sites on living cells using peak force tapping atomic force microscopy, Langmuir., 28 (2012), 16738-16744.
A. Beaussart, D. Alsteens, S. El-Kirat-Chatel, P.N. Lipke, S. Kucharíková, P. Van Dijck, Y.F. Dufrêne, Single-molecule imaging and functional analysis of Als adhesins and mannans during Candida albicans morphogenesis, ACS Nano., 12 (2012), 10950-10964.
C.Jimeńez-Ortigosa, V. Aimanianda, L. Muszkieta, I. Mouyna, D. Alsteens, S. Pire, R. Beau, S. Krappmann, A. Beauvais, Y.F. Dufrêne, C. Roncero, J.P. Latgé, Chitin synthases with a myosin motor-like domain control the resistance of Aspergillus fumigatus to echinocandins, Antmicrob. Agents Ch., 56 (2012), 6121-6131.
J.J Heinisch, P.N. Lipke, A. Beaussart, S. El Kirat Chatel, V. Dupres, D. Alsteens, Y.F. Dufrêne, Atomic force microscopy–looking at mechanosensors on the cell surface, J. Cell Sci., 125 (2012), 4189-4195.
D. Alsteens, C.B. Ramsook, P.N. Lipke, Y.F. Dufrêne, Unzipping a functional microbial amyloid, ACS Nano., 6 (2012), 7703-7711.
D. Alsteens, Microbial cells analysis by atomic force microscopy, Methods in Enzymology., 506 (2012), 3-17.
P.N. Lipke, M.C. Garcia, D. Alsteens, C.B. Ramsook, S.A. Klotz, Y.F Dufrêne, Strenghtening relationships: Amyloids create adhesion nanodomains in yeasts, Trends Microbiol., 20 (2012), 59-65.

2011

M.C. Garcia, J.T. Lee, C.B. Ramsook, D. Alsteens, Y.F. Dufrêne, P.N. Lipke, A role for amyloid in cell aggregation and biofilm formation, PLOS One., 6 (2011).
M. Ouberai, F. El Garch, A. Bussiere, M. Riou, D. Alsteens, L. Lins, I. Baussanne, Y. F. Dufrêne, R. Brasseur, J.L. Decout, M.P. Mingeot-Leclercq, The Pseudomonas aeruginosa membranes : a target for a new amphiphilic aminoglycoside derivative?, Biochim. Biophys. Acta-Biomembr., 6 (2011), 1716-1727.
D. Alsteens, V. Dupres, G. Andre, Y.F. Dufrêne, Frontiers in microbial nanoscopy, Nanomedicine., 6 (2011), 395-403.

2010

D. Alsteens, M.C. Garcia, P.N. Lipke, Y.F. Dufrêne, Force-induced formation and propagation of adhesion nanodomains in living fungal cells, Proc. Natl. Acad. Sci. USA., 107 (2010), 20744-20749.
V. Dupres, D. Alsteens, G. Andre, Y.F. Dufrêne, Microbial nanoscopy: a closer look at microbial cell surfaces, Trends Microbiol., 18 (2010), 397-405.
J.J. Heinisch, V. Dupres, D. Alsteens, Y.F. Dufrêne, Measurement of the mechanical behavior of yeast membrane sensors using single-molecule atomic force microscopy, Nat. Protoc., 5 (2010), 670-673.

2009

D. Alsteens, E. Dague, C. Verbelen, G. Andre, V. Dupres, Y.F. Dufrêne, Nanoscale imaging of microbial pathogens using atomic force microscopy, WIREs Nanomed. Nanobiotechnol., 1 (2009), 68-180.
D.J. Müller, M. Krieg, D. Alsteens, Y.F. Dufrêne, New frontiers in atomic force microscopy: analyzing interactions from single-molecules to cells, Curr. Opin. in Biotechnol., 20 (2009), 4-13.
D.J. Müller, J. Helenius, D. Alsteens, Y.F. Dufrêne, Force probing surfaces of living cells to molecular resolution. Nat. Chem. Biol., 5 (2009), 383-390.
V. Dupres, D. Alsteens, K. Pauwels, Y.F. Dufrêne, In vivo imaging of S-layer nanoarrays on Corynebacterium glutamicum, Langmuir., 25 (2009), 9653-9655.
V. Dupres, D. Alsteens, G. Andre, C. Verbelen, Y.F. Dufrêne, Fishing single molecules on live cells, Nanotoday., 4 (2009), 262-268.
V. Dupres, D. Alsteens, S. Wilk, B. Hansen, J.J. Heinisch, Y.F. Dufrêne, The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo, Nat. Chem. Biol., 5 (2009), 857-862.
D. Alsteens, E. Pesavento, G. Cheuvart, V. Dupres, H. Trabelsi, P. Soumillion, Y.F. Dufrêne, Controlled manipulation of bacteriophages using single-virus force spectroscopy, ACS Nano., 3 (2009), 3063-3068.
D. Alsteens, V. Dupres, S.A. Klotz, N.K. Gaur, P.N. Lipke, Y.F. Dufrêne, Unfolding individual Als5p adhesion proteins on live cells, ACS Nano., 3 (2009), 1677-1682.
G. Francius, D. Alsteens, V. Dupres, S. Lebeer, S. De Keersmaecker, J. Vanderleyden, H.J. Gruber, Y.F. Dufrêne, Stretching polysaccharides on live cells using single molecule force spectroscopy, Nat. Protoc., 4 (2009), 939-946.
C. Verbelen, N. Christiaens, D. Alsteens, V. Dupres, A.R. Baulard, Y.F. Dufrêne, Molecular mapping of lipoarabinomannans on mycobacteria, Langmuir., 25 (2009), 4324-4327.

2008

E. Dague, D. Alsteens, J.-P. Latgé, Y.F. Dufrêne, High-resolution cell surface dynamics of germinating Aspergillus fumigatus conidia, Biophys. J., 94 (2008), 656-660.
G. Francius, S. Lebeer, D. Alsteens, L. Wilding, H. J. Gruber, P. Hols, S. De Keersmaecker, J. Vanderleyden, Y. Dufrêne, Detection, localization, and conformational analysis of single polysaccharide molecules on live bacteria, ACS Nano., 2 (2008), 1921-1929.
D. Alsteens, C. Verbelen, E. Dague, D. Raze, A.R. Baulard, Y.F. Dufrêne, Organization of the mycobacterial cell wall: a nanoscale view, Pflüg. Arch. Eur. J. Phy., 456 (2008), 117-125.
D. Alsteens, V. Dupres, K. Mc Evoy, L. Wildling, H.J. Gruber, Y.F. Dufrêne, Structure, cell wall elasticity and polysaccharide properties of living yeast cells, as probed by AFM, Nanotechnol., 19 (2008).

2007

E. Dague, Y. Gilbert, C. Verbelen, G. Andre, D. Alsteens, Y.F. Dufrêne, Towards a nanoscale view of fungal surfaces, Yeast., 24 (2007), 229-237.
D. Alsteens, E. Dague, C. Verbelen, G. Andre, G. Francius, Y.F. Dufrêne, Nanomicrobiology, Nanoscale Res. Lett., 2 (2007), 365-372.
E. Dague, D. Alsteens, J.P. Latgé, C. Verbelen, D. Raze, A.R. Baulard, Y.F. Dufrêne, Chemical force microscopy of single live cells, Nano Lett., 7 (2007), 3026-3030.
D. Alsteens, E., Dague, P.G., Rouxhet, A.R., Baulard, Y.F. Dufrêne, Direct measurement of hydrophobic forces on cell surfaces using AFM, Langmuir., 23 (2007), 11977-11979.