Cell analysis: FACSCantoII™ and FACSCalibur™

FACSCantoII™

The analyzer BD FACSCantoII™ is one of the most versatile and intuitive flow cytometers thanks to BD FACSDiva™ software that automatically checks the configuration, acquisition and analysis. The automatic and off-line compensation process saves time to researchers. Recorded data can be analyzed in different ways and are easily identified through the hierarchical gates.

User Guide

Specifications

3 lasers, 8 fluorescences :

405nm: Pacific Blue, H450, BV421 / AmCyan, H500, BV510
488nm: FITC, Alexa-488, GFP, CFSE/ PE / PerCP, PE-Cy5.5, 7-AAD, PI / PE-Cy7
633nm: APC / APC-Cy7, APC-H7

Applications

Multiparametric Immunophenotype and rare events detection
TCR repertoire
Signal Transduction (PhosFlow) (Link1)
Cell cycle (Link1; Link2; Link3)
DNA/RNA content
Cell proliferation
Cell death
Mitochondria and cellular membrane potential alterations
mRNA and microRNA detection
Exosomes and microvesicles detection (Link1: Link2)

Training

Each researcher can use the instrument after following the course taught by Davide Brusa or demonstrating proficiency in the use of the same device.

Fees

	IREC	UCL	Private
FACSCantoII	20 €/h	30 €/h	on request

FACSCalibur™

The BD FACSCalibur™ platform allows users to perform cell analysis and four colours immunophenotype. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications.